Glycosylation of human corticosteroid-binding globulin at aspargine 238 is necessary for steroid binding.
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چکیده
منابع مشابه
Glycosylation of human corticosteroid-binding globulin at aspargine 238 is necessary for steroid binding.
Human corticosteroid binding-globulin (CBG) is a plasma glycoprotein that binds and regulates the biological activity of glucocorticoids and progesterone. Carbohydrates comprise approximately 25% of its molecular mass being represented by bi- and triantennary N-linked oligosaccharides of the N-acetyllactosamine type. To assess the impact of these carbohydrate chains on CBG production and steroi...
متن کاملFunctional implications of corticosteroid-binding globulin N-glycosylation
Corticosteroid-binding globulin (CBG) is a plasma carrier of glucocorticoids. Human and rat CBGs have six N-glycosylation sites. Glycosylation of human CBG influences its steroid-binding activity, and there are N-glycosylation sites in the reactive center loops (RCLs) of human and rat CBGs. Proteolysis of the RCL of human CBG causes a structural change that disrupts steroid binding. We now show...
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Specific binding sites for corticosteroid-binding globulin were detected on membranes prepared from human prostates. The binding sites are typical of membrane receptors: they are saturable and specific and have high affinity. There was little specific binding at 4 degrees C and 23 degrees C. Maximal specific binding was obtained at 37 degrees C. Scatchard analysis revealed the presence of a sin...
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BioBreeding (BB) rats are derived from an outbred colony of Wistar rats and are used as a model of autoimmune diabetes mellitus. A corticosteroid binding globulin (CBG) variant with reduced affinity for glucocorticoids has now been found in the blood of these animals. The dissociation rate constants of BB CBG for cortisol (4.42 nM) and corticosterone (1.43 nM) are both about 50% higher than tho...
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Corticosteroid-binding globulin was isolated from normal human plasma by successive anion exchange chromatography and gel filtration. Ammonium sulfate fractionation was employed as a preliminary step when large volumes were processed. The purification was about 4000-fold. Homogeneity was shown by free boundary, agar and paper electrophoresis, sedimentation velocity, and diffusion. Immunoelectro...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1993
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)54013-8